Recombinant antigen-based ELISA kit for diagnosis of Theileria equi infection (T. equi -ELISA kit)
Technology Details:
Equine piroplasmosis - also called equine babesiosis - is an acute, sub-acute or chronic tick-borne disease of equids, caused by intraerythrocytic protozoa, Theileria equi (T. equi) or Babesia caballi. Sero-prevalence of equine piroplasmosis is up to 35% in the indigenous equid population. Recently, NRCE has developed a recombinant antigen-based ELISA kit for the diagnosis of equine piroplasmosis.
Theileria equi -ELISA kit, is a recombinant erythrocyte merozoite antigen (EMA-2) based plate ELISA for the detection of antibodies against Theileria equi infection in equine. The kit has a ready-to-use ELISA plate onto which serum samples can be loaded after proper dilution. The results can be obtained in 1hr 30 min times after the application of serum samples. One ELISA plate has the capacity to test 42 samples in duplicate, with a reagents shelf life up to 6 months when kept at 4oC. The test sensitivity and specificity are comparable to OIE recommended ELISA. This kit is highly sensitive and helps in early detection of disease along with r-ELISA gave uniform results and no batch to batch antigen variation is observed.