Technology Details:
Coliforms are considered as faecal indicator organisms which indicate the presence of other potentially harmful, disease-causing organisms/pathogens in milk and milk products. Technology for detection of coliforms has been developed involving targeting enzyme substrate reaction for specific marker enzyme (s) to release free chromogen which can be visually detected by a color change within 12.15±0.30h of incubation employing CSM as a selective medium in lyophilized form. The developed assay is of immense importance for the food industry in rapid detection of Coliforms which otherwise are monitored by conventional ISO 4832:2006 methods requiring 48h using plate method / or ISO 831:2006 requiring 72h using MPN method. The novel features of the technology are as follows: The appearance of yellow color confirms the presence of coliforms. The developed enzyme assay for coliforms can confirm <1.0 log CFU/ml within 12.15±0.30 h of incubation against 2-3 days protocol following conventional method. Selective inhibition of non-coliforms likes Salmonella, Shigella, Yersinia, and Proteus. The wide scope of application to raw, pasteurized and dried milk for routine as well as for regulatory standard compliance. Lab Validation of developed kit with ISO 4832:2006 using raw, pasteurized and dried milk.