Initiation of lactation places one of the greatest stresses on Ca homeostasis and is associated with milk fever among high producing dairy cows and buffaloes. Hypocalcaemia also increases the incidence of dystocia, retained placenta, metritis, prolapsed uterus and delays uterine involution. Hypocalcaemia prevents the teat sphincter from closing both before and after milking and allows greater access to bacteria in the mammary gland which results in mastitis. During dry period, Ca requirement is 10-12 g/d hence mechanism for replenishing plasma Ca is relatively inactive. But upon parturition, Ca requirements are 10 times more than the supply in bloodstream which cannot be met just by increasing the dietary level of Ca. Feeding of slightly negative-charged ration to pregnant cows/buffaloes at least 3 weeks before parturition creates metabolic acidosis and initiate Ca resorption from the bones thereby fulfilling the increased demands of Ca and blood Ca levels are maintained. Technology Details: Supplementation of diet of animal with special kind of mineral mixture is effective for the prevention of hypocalcaemia, minimizing the occurrence of milk fever and the many metabolic disorders that accompany this condition. Apart from manipulation of cation anion balance, vitamin E is additionally added to the mineral mixture. It is well established that transition animals experience extensive oxidative stress which is a contributing factor to increased susceptibility to a variety of disorders and poor reproductive performance. The supplementation of vitamin E can be useful against oxidative stress in per parturient dairy cows.