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A strip-based test for detection of sucrose in milk

Technology Details:
Sucrose is not a permitted additive in milk as per FSSAI rules. Sucrose is added in the milk to fraudulently increase its solids–not–fat content. A rapid strip-based test for detection of sucrose in milk has been developed. The developed strip test can detect 0.1% sucrose in milk. The test involves dipping the strip in milk and observing the change in colour after 5 min. The strip is white in colour and in case of milk adulterated with sucrose changes to pink colour. The intensity of developed pink colour is proportional to extent of sucrose in milk. In case of pure milk, the strip retains its original white colour. The developed detection strip can also be used to detect the presence of sucrose in various milk products (Khoa, Dahi etc.). The colour change in sucrose detection strip also occurs in presence of glucose or H₂O₂. Hence, the presence of sucrose will be confirmed only in absence of H₂O₂ and glucose which can be ascertained by H₂O₂ and glucose detection strips, respectively. The developed test is more sensitive than the existing liquid-based test. The shelf-life is more than 1 month at refrigeration temperature. The test can be performed even by non-technical person. The test is convenient to do and can be easily done at milk collection centres, reception dock of dairy industries as well as at house hold levels.