Background:
Bovine leukocyte adhesion deficiency (BLAD) is an autosomal recessive congenital disease characterized by recurrent bacterial infections, delayed wound healing and stunted growth, and is also associated with persistent marked neutrophilia. Affected cattle die at an early age due to the infectious complications. The guidelines of Central Monitoring Unit (CMU) stipulate that the bulls used for breeding should be tested for four genetic disorders including BLAD to avoid its transmission to its progeny. Technology Details:
The CIRC-Cattle BLAD Diagnostic assay kit is used for early detection of Bovine Leukocyte Adhesion Deficiency (BLAD) carrier animals. The test is earmarked with its Rapidity (can be completed within 2-3 hours), user friendliness (as it is easy to perform), Economical (because it does not need expensive equipment and reagents) and Specificity (as ensured by the deliberate mismatch introduced into the primers). The kit may prove to be an effective tool for selecting BLAD free animals especially young bulls at their early stage of life. This kit will be useful for the semen collection/germplasm centres, research institutions, State Animal Husbandry Departments and NGOs involved in semen collection and processing of cattle and buffaloes.