Technology Details:
Rotaviruses are the major etiologic agents of severe, acute dehydrating diarrhoea resulting in high morbidity and mortality in neonatal animals (including foals) and human infants. A monoclonal antibody (mAb)-based sandwich enzyme immunoassay (ELISA) has been developed for the diagnosis of rotavirus in different animals and human stool samples. This kit employs a polyclonal anti-rotavirus serum as a coating antibody to capture the rotavirus antigen and a monoclonal antibody is used for the detection of group-specific antigen present on the captured rotaviruses. The mAb raised against group-specific protein VP6 of rotavirus, for detection of mammalian group A rotaviruses. Higher colour intensities in comparison to the negative sample confirm the presence of rotavirus in the samples. The performance of Equip Rotavirus Kit was compared with that of virus isolation alone and the kit gave 100% sensitivity and a specificity of 96%. Thus, this kit will prove helpful in the timely diagnosis of rotavirus associated diarrhoea in the animal and human population.