Background:
Classical Swine Fever (CSF), Japanese Encephalitis (JE) and Porcine Reproductive and Respiratory Syndrome virus (PRRSV) are three important RNA viral pathogens of swine. Hence a simple, sensitive, and specific diagnostic approach is required to distinguish the viral pathogens. Currently, no convenient and specific multiplexing diagnostic assay kits for the simultaneous detection of these pathogens are available.:
Technology Details:
To detect CSF, JE, and PRRS in porcine samples, a specific set of oligonucleotide primers was designed and combined in a multiplex arrangement. To demonstrate the accuracy of the developed multiplex PCR assay, 413 porcine post-mortem tissue samples were tested. Each of the CSFV, JEV, and PRRSV target genes was amplified using the designed primers, but no other porcine virus genes were detected. The detection limit of the assay was 10²-10³ copies/µl of viral nucleic acid at the picogram level. The benefit of the assay over existing technologies: Cost-effective: Since no expensive equipment is required; No containment facilities are required; Can be performed in any laboratory; and Simultaneously tests for the presence of all three RNA viruses.