Technology Details:
Milk contains low amount of natural glucose (less than 5 mg/100 ml). Milk is adulterated with glucose (pure or crude form which is by-product of other industry) to fraudulently increase its solids-not-fat content. As per FSSAI, glucose is not a permitted additive in milk. A rapid paper-based strip test has been developed for the detection of glucose in milk. The prepared strip is white in colour. The test involves dipping the strip in milk followed by visualization of change in colour of the strip. The colour change to pink after about 5 minutes in case of milk is adulterated with glucose. The intensity of pink colour produced in the strip is proportional to the amount of glucose present in milk sample. The test can detect presence of 0.02% level of glucose in milk. The developed detection strip can also be used to detect the presence of glucose in various milk products (Khoa, Dahi etc.). The colour change in glucose detection strip also occurs in presence of glucose or H₂O₂. Hence, the presence of glucose will be confirmed only in absence of H₂O₂-which can be ascertained by H₂O₂ detection strip. The developed test is more sensitive than the existing liquid-based test. The shelf-life is more than 3 months at refrigeration temperature. The test can be performed even by non-technical person. The method has been validated at a NABL accredited laboratory. The test can be used at milk collection center, reception dock of dairy industries and also at house hold levels.