Technology Details:
The TaqMan® Real-time PCR-based Multi-target technology consists of a 96-well prespotted plate with lyophilized primers and probes, for simultaneous detection of 47 targets in duplicate, including 21 event-specific sequences, 5 construct regions, 15 for transgenic elements, and 6 taxon-specific targets for cotton, eggplant, maize, potato, rice, and soybean. Limit of detection (LOD) of assays ranged from 0.1 to 0.01% GM content for different targets. This GMO screening technology allows detection of Indian GM events/crops that are authorized for commercial cultivation, or in confined field trials and imported for research purposes.