Background JEV causes acute encephalitis syndrome in humans and reproductive disorders viz. stillbirth, abortion, mummified fetuses, and orchitis in pigs. Since it is a zoonotic disease and the pigs are the amplifiers of the virus, there is a necessity to develop a vaccine intended for vaccinating pigs in the endemic areas to break the virus transmission cycle. Technology Details The purpose of the present invention was to develop an indigenous cell culture-based inactivated Japanese encephalitis vaccine from an Indian JEV isolates. Since the vaccine is prepared from swine JEV isolate (JEV/SW/IVRI/395A/2014), it is expected that the vaccine would be able to protect the pigs from prevalent JEV strains in the country. It is expected to reduce the disease burden in the pig and human population.