Background:
Due to its extremely high fatality rate, African Swine Fever (ASF, which is regarded as the most terrifying swine disease first appeared in India in 2020. Hence, the development of indigenous rapid and reliable diagnostics is always demanding in the present scenario.:
Technology Details:
Here we developed an in-house reliable, sensitive, and specific quantitative assay for the detection of ASV in clinical specimens. The developed assay can be able to detect the ASF viral load in clinical samples within 30 minutes (post nucleic acid extraction). Disclosed herein are the primers and probe sequences specific for the detection of African swine fever virus (ASFV) in clinical samples comprising of a) forward primer (Seq 1). b) reverse primer (Seq 2), c) probe sequence (Seq 3) comprising 23 bases labeled with ROXTM (at 5 ends) & BHQTM (at 3 ends). Further, the disclosure is a process for quantitative detection of ASFV copy numbers in clinical samples within 30 minutes using the seq 1-3. The assay is specific for ASFV without any cross-reactivity with other swine viral pathogens. The assay was found to detect as low as 5 femtograms of ASF viral DNA.