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Construct-specific Visual LAMP for construct regions: p35S-cry1Ac, cry2Ab2-tnos for insect resistance, and cp4epsps-tnos for herbicide tolerance

Technology Details:
Construct-specific Visual LAMP technology targeting p35S-cry1Ac, and cry2Ab2-tnos for insect resistance efficiently detects the target construct regions with the limit of detection (LOD) as low as 0.01%. It allows user-friendly, rapid and cost-efficient detection of Bt events with target construct regions, where positive amplification is detected visually with change in colour from orange to green, without requiring cumbersome time-consuming gel electrophoretic analysis. Visual LAMP technology targeting p35S-cry1Ac allows checking for presence of four approved Bt cotton events, MON531, MON15985, GFM and Event 1, whereas assay targeting cry2Ab2-tnos allows specific detection of stacked GM event MON15985.