Background:
The vitamin-producing GRAS status lactobacilli can be used as an alternative to chemically vitamins fortified products and would permit the production of foods with elevated concentrations of vitamins that are less likely to cause undesirable side-effects. The vitamin B12 producing Lactobacillus reuteri NCDC 958/VTCC610B has been isolated from the infants’ feces. L. reuteri NCDC 958/VTCC610B endowed with extracellular vitamin B12 production that was observed to be 132.21±1.94 ng/mL in vitamin B12 deficient medium. L. reuteri NCDC 958/VTCC610B was robust to a host of environmental stresses and also showed good in vitro probiotics and safety attributes. The vitamin B12 production by L. reuteri NCDC 958/VTCC610B was 145.60±3.49 ng/mL in optimized soy-curd. The vitamin B12 bio-fortified soya curd fermented by NCDC 958 showed antimicrobial activity, and other bio-functional property such as DPP IV inhibitory activity, ACE-inhibitory activity, and antioxidative activity. Furthermore, the vitamin B12 produced by NCDC 958 was observed to be bioavailable in vitamin B12 deficient rat model. The vitamin B12 bio-fortified fermented soya curd fed rat group showed the reduction in death of pups, reduction in pro-inflammatory cytokines i.e. IL-6 and TNF-alpha levels, and improvement of anti-oxidant enzymes. The functional property of vitamin B12 bio-enriched soy cured fermented by L. reuteri NCDC 958/VTCC610B indicated its potential for commercial adaption to combat vitamin B12 deficiency
Technology Details:
The vitamin-producing GRAS status lactobacilli can be used as an alternative to chemically vitamins fortified products and would permit the production of foods with elevated concentrations of vitamins that are less likely to cause undesirable side-effects.