Background:
GPx family is the key enzyme for the detoxification of ROS in aquatic organisms, including fish. GPx1 (classical GPx) is a selenoprotein, first discovered and most abundantly expressed in the GPx family. GPx-based DNA vaccine has been found to effective against Vibrio harveyi infection in fish. It has been showed that GPx acted as a major enzymatic defense system in the infectious parasites in order to ensure their survival from the harmful exposure to ROS metabolism in the host. Hence, the defense against the oxidative damage is essential for the parasite to survive, which indicated GPx could be an effective antigen against the parasites. For generation of these types of basic knowledge, there is unavailability of fish specific proteins and antibodies Technology Details:
Rohu GPX-1 was found to be having antioxidant property. It’s presence in serum at low level could be easily detected by standardized ELISA using this produced polyclonal antibody and the recombinant protein generated in this technology. Further, the clone could be of further use for vaccine candidate screening against important bacteria and parasites of fish.