Background:
Lysozyme, an important secretory innate immune component, possesses antimicrobial activity against broad spectrum of bacteria and viruses. The lytic activities of the Lysozyme G against Gram-positive and Gram-negative bacteria have been reported in many fish species. Lysozyme also causes viral deactivation by directly binding to negatively charged virus protein, DNA, RNA and apoprotein of virus. Besides antimicrobial activity, lysozymes also play a major role in digestion, reproduction and growth stimulation, and having also anti-inflammatory, anti-tumor and immunomodulatory roles. Currently there is no such product available in India or world-wide from Indian major carps like rohu, either for its estimation in blood by ELISA or use of this protein as broad-spectrum antimicrobial agent. Technology Details:
Rohu lysozyme G was found to be having lytic property against both Gram positive and negative bacteria. It’s presence in serum at low level could be easily detected by standardized ELISA using this produced polyclonal antibody and the recombinant protein generated in this technology. With an era of increasing antimicrobial resistance pattern to conventional synthetic antibiotics, this fish recombinant protein could be a potential alternative to antibiotics.