Background:
The glutathione S-transferases (GSTs) are a multifunctional family of phase II enzymes that detoxify a variety of environmental chemicals, reactive intermediates, and secondary products of oxidative damage. GST mRNA expression and catalytic activity have been used as biomarkers of exposure to environmental chemicals. However, factors such as species differences in induction, partial analyses of multiple GST isoforms, and lack of understanding of fish GST gene regulation, have confounded the use of GSTs as markers of of oxidative stress. However, lack of availability of species specific proteins and assay system pose a serious concern in its estimation or further analysis in different stressful conditions in fish. Technology Details:
GST(mu) was found to be having role in detoxification of chemical cures in aquaculture. It’s presence in serum at low level could be easily detected by standardized ELISA using this produced polyclonal antibody and the recombinant protein generated in this technology. As it is very species-specific, the recombinant protein-based ELISA would be able to measure its activity at low level.